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Specific recognition of cruciform DNA by nuclear protein HMG1
M E Bianchi1, M Beltrame, G Paonessa
1European Molecular Biology Laboratory, Heidleberg, Federal Republic of Germany.
Science (New York, N.Y.)
|February 24, 1989
Abstract:
Cruciform DNA, a non-double helix form of DNA, can be generated as an intermediate in genetic recombination as well as from palindromic sequences under the effect of supercoiling. Eukaryotic cells are equipped with a DNA-binding protein that selectively recognizes cruciform DNA. Biochemical and immunological data showed that this protein is HMG1, an evolutionarily conserved, essential, and abundant component of the nucleus. The interaction with a ubiquitous protein points to a critical role for cruciform DNA conformations.