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The Kinase STK3 Interacts with the Viral Structural Protein VP1 and Inhibits Foot-and-Mouth Disease Virus Replication

Huisheng Liu1, Qiao Xue1, Qiaoying Zeng1

  • 1Laboratory of Veterinary Microbiology, College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, China.

Biomed Research International
|December 12, 2017
PubMed

Insights

We discovered that serine/threonine kinase 3 (STK3) interacts with foot-and-mouth disease virus (FMDV) protein VP1. STK3 inhibits FMDV replication, offering potential antiviral targets.

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Foot-and-mouth disease virus (FMDV) causes significant losses in livestock.
  • The FMDV structural protein VP1 is crucial for pathogenesis, but its host interactions are not fully understood.

Purpose of the Study:

  • To identify host cell proteins interacting with FMDV VP1.
  • To investigate the role of these interactions in FMDV replication.

Main Methods:

  • Yeast two-hybrid system to screen for interacting proteins.
  • Coimmunoprecipitation assays to confirm VP1-STK3 interaction in cell lines.
  • Overexpression and knockdown of STK3 to assess its effect on viral replication.

Main Results:

  • Identified serine/threonine kinase 3 (STK3) as a binding partner of FMDV VP1.
  • Confirmed the interaction between VP1 and STK3 in human and porcine kidney cells.
  • Demonstrated that STK3 significantly inhibits FMDV replication in porcine kidney cells.

Conclusions:

  • STK3 plays a role in restricting FMDV replication.
  • This study reveals novel host cell kinase involvement in FMDV infection.
  • STK3 represents a potential target for developing antiviral strategies against FMDV.

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