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ST2 gene products critically contribute to cellular transformation caused by an oncogenic Ras mutant

Kenji Tago1, Satoshi Ohta1, Masaki Kashiwada2

  • 1Division of Structural Biochemistry, Department of Biochemistry, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan.

Heliyon
|December 12, 2017
PubMed

Insights

The ST2 gene product accelerates Ras-induced cellular transformation by affecting cell cycle progression. This study reveals a novel mechanism for ST2

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Oncology

Background:

  • The ST2 gene is a primary responsive gene induced by growth factors and oncogenic stresses.
  • ST2 and ST2L proteins are involved in cellular responses to various stimuli.

Purpose of the Study:

  • To investigate the role of ST2 gene products in oncogenic Ras-induced cellular transformation.
  • To elucidate the mechanism by which ST2 influences cell cycle progression during transformation.

Main Methods:

  • Utilized oncogenic Ras mutant (Ras (G12V)) in NIH-3T3 murine fibroblasts.
  • Employed enforced expression and RNA-interference to modulate ST2 gene product levels.
  • Assessed cellular transformation and Rb phosphorylation.

Main Results:

  • Oncogenic Ras mutant induced ST2 and ST2L protein expression.
  • Enforced ST2 expression enhanced Ras (G12V)-induced cellular transformation.
  • ST2 gene silencing suppressed Ras (G12V)-induced transformation and reduced Rb phosphorylation.

Conclusions:

  • Oncogenic Ras-induced ST2 expression is crucial for accelerating cellular transformation, independent of IL-33.
  • ST2 gene products play a significant role in cell cycle progression during transformation.
  • A novel mechanism for ST2's involvement in cellular transformation and proliferation is suggested.
Keywords:
Cancer researchCell biology

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