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Essential Metal Uptake in Gram-negative Bacteria: X-ray Fluorescence, Radioisotopes, and Cell Fractionation
Published on: February 1, 2018
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Metal-citrate complex transport in Kineococcus radiotolerans
Brian P Huta1, Nigel H Miller1, Eleanor L Robertson1
1Department of Chemistry, Syracuse University, Syracuse, New York, USA.
Journal of Basic Microbiology
|December 12, 2017
Summary
Kineococcus radiotolerans utilizes a permease mechanism to transport calcium-citrate, essential for organism growth. This study confirms the bacterium
Area of Science:
- Microbiology
- Biochemistry
- Molecular Biology
Background:
- Organismal growth relies on acquiring carbon and metal nutrients.
- The CitMHS superfamily facilitates simultaneous nutrient uptake.
- A CitMHS gene was identified in Kineococcus radiotolerans.
Purpose of the Study:
- To investigate citrate transport in Kineococcus radiotolerans.
- To determine the role of metal ions in citrate uptake.
- To characterize the transport mechanism and its environmental dependencies.
Main Methods:
- Bioinformatic analysis to identify the CitMHS gene.
- Radiotracer flux assays using 14-C radiolabeled citrate.
- Testing citrate uptake with various metal ion complexes and pH conditions.
Main Results:
- Kineococcus radiotolerans internalizes citrate when complexed with specific metal ions, notably calcium.
- Citrate uptake is pH-dependent, with higher rates at pH 7 than pH 10.
- The transport mechanism is consistent with an ATP-independent permease.
Conclusions:
- Kineococcus radiotolerans possesses the capability to transport metal-citrate complexes.
- Calcium-citrate is the primary form of citrate internalized by the organism.
- The identified CitMHS protein mediates efficient citrate acquisition in response to environmental cues.
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