Detection of depth-depend changes in porcine cartilage after wear test using Raman spectroscopy

Cartilage damage and wear can lead to severe diseases, such as osteoarthritis, thus, many studies on the cartilage wear process have already been performed to better understand the cartilage wear mechanism. However, most characterization methods focus on the cartilage surface or the total wear extent. With the advantages of high spatial resolution and easy characterization, Raman microspectroscopy was employed for the first time to characterize full-depth changes in the cartilage extracellular matrix (ECM) after wear test. Sections from the cartilage samples after wear were compared with sections from the control group. Univariate and multivariate analyses both indicated that collagen content loss at certain depths (20%-30% relative to the cartilage surface) is possibly the dominating alteration during wear rather than changes in collagen fiber orientation or proteoglycan content. These findings are consistent with the observations obtained by scanning electron microscopy and histological staining. This study successfully used Raman microspectroscopy efficiently assess full-depth changes in cartilage ECM after wear test, thus providing new insight into cartilage damage and wear.