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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Feb 17, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Visualizing Single-Cell Secretion Dynamics with Single-Protein Sensitivity.

Matthew P McDonald¹, André Gemeinhardt¹, Katharina König^1,2

¹Nano-Optics Division, Max Planck Institute for the Science of Light , Staudtstraße 2, 91058 Erlangen, Germany.

|December 12, 2017

Summary

This summary is machine-generated.

Researchers developed a new method to track single unlabeled proteins secreted by living cells in real-time. This technique, interferometric scattering (iSCAT), revealed that Laz388 cells release IgG antibodies and other viral proteins at high rates.

Keywords:

cellular secretion dynamics iSCAT imaging label-free single-protein

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Area of Science:

Biophysics
Cell Biology
Immunology

Background:

Cellular protein secretion is vital for biological processes like communication and immune response.
Existing methods often require labeling or lack real-time, single-molecule sensitivity.

Purpose of the Study:

To develop and demonstrate a novel, label-free method for real-time detection and imaging of single secreted proteins from living cells.
To quantify protein secretion rates from individual cells.

Main Methods:

Utilized interferometric scattering (iSCAT) to detect scattered light from unlabeled proteins.
Applied the technique to Laz388 cells, an Epstein-Barr virus (EBV)-transformed B cell line.
Demonstrated application to single-cell lysate analysis.

Main Results:

Achieved real-time, single-protein sensitivity imaging of secreted proteins.
Quantified IgG antibody secretion from single Laz388 cells at approximately 100 molecules per second.
Observed co-secretion of other proteins/particles (100 kDa-1 MDa) with IgG, potentially EBV-related.

Conclusions:

Label-free iSCAT imaging is a powerful tool for studying real-time cellular secretion.
Provides new insights into the dynamics of protein exchange between cells and their environment.
Enables high-sensitivity analysis of cellular secretions and lysates.