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Arabidopsis thaliana Polar Glycerolipid Profiling by Thin Layer Chromatography TLC Coupled with Gas-Liquid Chromatography GLC
Published on: March 18, 2011
[STUDY OF SOME PLANT SEED LIPIDS GROWING IN GEORGIA]
B Kikalishvili1, N Gorgaslidze1, Ts Sulakvelidze1
1Tbilisi State Medical University, I. Kutateladze Institute of Pharmacochemistry, Georgia.
Abstract:
The purpose of the research was to study seed lipids of cultivated plants: Medicago sativa, Ruta graveolens L., Linum usitatissimum L., which are growing on experimental field of I. Kutateladze Institute of Pharmacochemistry. From the above-mentioned plant seeds was obtained the sum of neutral and polar lipids, the classes were determined qualitatively. Using standard methods were determined some physico-chemical constant of oils. Fatty acids was established qualitatively and was determined quantitatively using Gas chromatography and High performance liquid chromatography methods. As a result of research in the seed oil of Medicago Sativa was revealed high percentage content of Oktadekadien - 9,12 fatty acid. Oktadekadien -9 dominates in the oil obtained from the seeds of Ruta graveolens L. and Oktadekantrien - 9,12,15 dominates in the oil obtained from the seeds of Linum usitatissimum L. Phospholipids were established qualitatively and using spectrophotometric method were determined In the sum of polar lipids: (λ 820 nm). The oils which are obtained from above-mentioned plant seeds contains biologically active compounds, which gives the opportunity to use these oils as in medical practice, also in cosmetology.
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