Ultrasonic Modified Sweet Potato Pectin Induces Apoptosis like Cell Death in Colon Cancer (HT-29) Cell Line
Fredrick Onyango Ogutu1,2, Tai-Hua Mu1, Hongnan Sun1
1a Laboratory of Fruit and Vegetable Processing , Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Ministry of Agriculture , Beijing , P.R. China.
Background:
Pectin and especially modified citrus pectin possesses anticancer activity. Hence, the current study investigated anticancer activity of ultrasonic-modified sweet potato pectin (SPP) on HT-29 cells to assess its potential as a cancer therapeutic agent.
Method:
The effect of ultrasonic treatment on SPP molecular weight, galacturonic acid content, degree of methoxylation, and neutral sugar was investigated. Moreover, the effect of sonicated variant on human HT-29 cell proliferation was assessed by MTT assay, cell cytotoxicity, and apoptosis by Annexin V/PI flow cytometer and caspase-3 activity was studied.
Results And Discussion:
Sonication led up to seven-fold decrease in molecular weight. The degree of methoxylation (DM) decreased more than two-fold. Moreover, the galacturonic acid (GalA) content increased up to 92%, arabinose and galactose content increased. The SSPP inhibited cell proliferation with the IC50 values 0.5 mg/ml and 0.75 mg/ml for 400 W and 200 W SSPP, respectively. Moreover, 14.41 ± 1.64% cell cytotoxicity was elicited by 400 W SSPP and 6.83 ± 0.80% by 200 W SSPP. Both SSPPs induced apoptosis with 400 W SSPP eliciting 19.42% and 42.21% apoptosis at 0.1 and 0.5 mg/ml, while 200 W SSPP induced 13.79% and 39.50% apoptosis at 0.1 and 0.5 mg/ml, respectively. SSPP activity increased with both increased concentration and sonication intensity.
Insights
Ultrasonic-modified sweet potato pectin (SPP) shows anticancer potential by inhibiting HT-29 cell proliferation and inducing apoptosis. Higher sonication intensity and concentration enhance SPP
Area of Science:
- Biochemistry
- Cell Biology
- Food Science
Background:
- Pectin, particularly modified citrus pectin, exhibits anticancer properties.
- Sweet potato pectin (SPP) was investigated for its anticancer effects on HT-29 cells.
- This study aimed to evaluate SPP as a potential cancer therapeutic agent.
Purpose of the Study:
- To investigate the anticancer activity of ultrasonic-modified sweet potato pectin (SPP) on HT-29 cells.
- To assess the impact of ultrasonic treatment on SPP's physicochemical properties.
- To determine the efficacy of modified SPP as a cancer therapeutic agent.
Main Methods:
- Ultrasonic treatment was applied to modify SPP.
- Physicochemical properties (molecular weight, galacturonic acid content, degree of methoxylation, neutral sugar) were analyzed.
- HT-29 cell proliferation (MTT assay), cytotoxicity, apoptosis (Annexin V/PI flow cytometry), and caspase-3 activity were assessed.
Main Results:
- Sonication significantly reduced SPP molecular weight and degree of methoxylation, while increasing galacturonic acid content.
- Modified SPP inhibited HT-29 cell proliferation, with IC50 values of 0.5 mg/ml (400 W) and 0.75 mg/ml (200 W).
- Modified SPP induced significant cell cytotoxicity and apoptosis in a dose-dependent manner, with enhanced effects at higher sonication intensities.
Conclusions:
- Ultrasonic modification alters SPP's physicochemical properties, enhancing its anticancer potential.
- Modified SPP effectively inhibits cancer cell proliferation and induces apoptosis.
- SPP modified by sonication demonstrates promise as a natural anticancer agent.
