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LncRNA as ceRNAs may be involved in lactation process
Shuai Yu1, Yong Zhao1, Fangnong Lai1
1College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, P. R. China.
Oncotarget
|December 13, 2017
Summary
Competing endogenous RNAs (ceRNAs) regulate goat mammary gland functions during lactation. These RNA interactions are crucial for milk production, particularly lipid and protein synthesis in mature lactation.
Area of Science:
- Molecular Biology
- Animal Science
- Genomics
Background:
- Mammary gland function is vital for milk production, regulated by complex molecular factors.
- Long non-coding RNAs (lncRNAs) are implicated in mammary gland development.
- Competing endogenous RNAs (ceRNAs) represent a regulatory network involving lncRNAs, microRNAs (miRNAs), and messenger RNAs (mRNAs).
Purpose of the Study:
- To investigate the role of ceRNAs in regulating goat mammary gland function during different lactation stages.
- To identify lncRNA-mRNA interactions and their association with lactation physiology.
Main Methods:
- RNA sequencing (mRNA, miRNA, lncRNA) of goat mammary tissue during early and mature lactation.
- Co-expression analysis of lncRNAs, miRNAs, and mRNAs.
- Bioinformatic analysis to identify ceRNA networks and associated pathways.
Main Results:
- ceRNAs upregulated during mature lactation are linked to lipid, protein, carbon, and amino acid metabolism.
- ceRNAs upregulated during early lactation are associated with PI3K-AKT signaling and ECM-receptor interactions, preparing the gland for lactation.
- Evidence suggests synergistic action of ceRNAs across lactation stages to control milk production.
Conclusions:
- ceRNAs play a significant role in regulating specific functions during different lactation stages in goats.
- The lncRNA-mRNA ceRNA network is potentially involved in the intricate process of lactation control.
- Findings highlight ceRNAs as key regulators of mammary gland function and milk synthesis.
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