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LncRNA and mRNA profiling during activation of tilapia macrophages by HSP70 and Streptococcus agalactiae antigen

Honglin Luo1,2, Huizan Yang1,3, Yong Lin1

  • 1Guangxi Key Laboratory for Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Institute of Fishery Sciences, Nanning, P.R. China.

Oncotarget
|December 13, 2017
PubMed
Abstract

Insights

This study profiles long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in tilapia peritoneal macrophages (TPMs) activated by heat shock protein 70 (HSP70) and Streptococcus agalactiae (Sa). It reveals distinct characteristics of lncRNAs and their regulatory roles in macrophage activation.

Area of Science:

  • Immunology
  • Molecular Biology
  • Genomics

Background:

  • Tilapia peritoneal macrophages (TPMs) play a crucial role in fish immunity.
  • Understanding the molecular mechanisms of TPM activation is vital for aquaculture health.
  • Long non-coding RNAs (lncRNAs) are increasingly recognized as key regulators in cellular processes.

Purpose of the Study:

  • To investigate the lncRNA and mRNA expression profiles during tilapia peritoneal macrophage activation.
  • To explore the regulatory relationships between lncRNAs and mRNAs in activated TPMs.
  • To identify key lncRNAs and mRNAs involved in the immune response of tilapia.

Main Methods:

  • RNA sequencing was employed to analyze lncRNA and mRNA profiles in TPMs stimulated with Streptococcus agalactiae (Sa) antigen, heat shock protein 70 (HSP70), and a combination of both.
  • Quantitative real-time PCR (qPCR) was used to validate the expression levels of selected lncRNAs and mRNAs.
  • Bioinformatic analyses were performed to identify co-located and co-expressed lncRNA-mRNA networks and potential competing endogenous RNA (ceRNA) interactions.

Main Results:

  • A significant number of lncRNAs (356), mRNAs (10173), and transcripts of uncertain coding potential (TUCPs) (1782) were differentially expressed.
  • lncRNAs exhibited distinct characteristics compared to mRNAs, being shorter, having fewer exons, and higher expression levels.
  • Co-location and co-expression analyses revealed intricate networks between lncRNAs and mRNAs, with seven specific mRNAs identified in networks with two particular lncRNAs.
  • Differentially expressed genes were associated with critical signaling pathways, including phosphorylation, cytokine-cytokine receptor interaction, endocytosis, and MHC protein complex.
  • ceRNA networks involving specific lncRNAs and mRNAs were identified, suggesting complex regulatory mechanisms.

Conclusions:

  • This study provides the first comprehensive expression profile of lncRNAs and mRNAs in tilapia macrophages activated by HSP70 and Sa.
  • The findings highlight the significant role of lncRNAs in regulating the immune response of tilapia macrophages.
  • The identified lncRNA-mRNA interactions and pathways offer insights into the molecular basis of tilapia immunity and potential targets for disease resistance.
Keywords:
HSP70Streptococcus agalactiaelong non-coding RNAmacrophagesoreochromis niloticus

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