Related Experiment Video

Updated: Feb 17, 2026

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
06:54

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Published on: October 27, 2020

14.5K

MiR-876-5p suppresses epithelial-mesenchymal transition of lung cancer by directly down-regulating bone morphogenetic

Liang Bao1, Lei Lv, Jinping Feng

  • 1Department of Respirology, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi 214002, Jiangsu Province, China.

Journal of Biosciences
|December 13, 2017
PubMed

Insights

MicroRNA-876-5p suppresses lung cancer progression by targeting bone morphogenetic protein 4 (BMP-4). Lower microRNA-876-5p and higher BMP-4 levels correlate with increased cancer cell migration, invasion, and metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Lung cancer is a leading cause of cancer mortality worldwide.
  • Epithelial-mesenchymal transition (EMT) is a critical process in lung cancer progression and metastasis.
  • MicroRNAs play crucial roles in regulating gene expression and cellular processes.

Purpose of the Study:

  • To investigate the role of microRNA-876-5p and its target bone morphogenetic protein 4 (BMP-4) in lung cancer EMT.
  • To determine the molecular mechanism by which microRNA-876-5p affects lung cancer cell behavior.
  • To explore the therapeutic potential of microRNA-876-5p and BMP-4 in lung cancer treatment.

Main Methods:

  • Analysis of microRNA-876-5p and BMP-4 expression in lung cancer cells and tissues.
  • Luciferase activity assay to confirm direct targeting of BMP-4 mRNA by microRNA-876-5p.
  • In vitro assays for cell migration, invasion, and EMT markers.
  • In vivo xenograft mouse model to assess the role of microRNA-876-5p and BMP-4 in metastasis.

Main Results:

  • MicroRNA-876-5p expression was decreased, while BMP-4 expression was increased in lung cancer.
  • MicroRNA-876-5p directly targets and inhibits BMP-4 mRNA expression.
  • MicroRNA-876-5p suppressed lung cancer cell migration, invasion, and EMT in vitro.
  • MicroRNA-876-5p inhibited lung cancer metastasis in vivo, dependent on BMP-4 expression.

Conclusions:

  • MicroRNA-876-5p acts as a tumor suppressor in lung cancer by down-regulating BMP-4.
  • The microRNA-876-5p/BMP-4 axis is a key regulator of EMT and metastasis in lung cancer.
  • MicroRNA-876-5p and BMP-4 represent potential therapeutic targets for lung cancer treatment.

More Related Videos

Induction and Analysis of Epithelial to Mesenchymal Transition10:37

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

36.6K
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells11:42

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Published on: April 7, 2017

9.9K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
6.7K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.1K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.6K