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Related Experiment Video

Updated: Feb 17, 2026

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
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Real-time two-photon lithography in controlled flow to create a single-microparticle array and particle-cluster array

Bing Xu1, Yang Shi, Zhaoxin Lao

  • 1CAS Key Laboratory of Mechanical Behavior and Design of Materials, Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei 230026, China. huyl@ustc.edu.cn dongwu@ustc.edu.cn.

Lab on a Chip
|December 13, 2017
PubMed
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We developed a novel real-time two-photon lithography method for 100% efficient trapping of microparticles in arrays. This technique enables precise particle manipulation for advanced microfluidic and optofluidic applications.

Area of Science:

  • Microfluidics
  • Optofluidics
  • Biomedical Engineering
  • Materials Science

Background:

  • Microarray technology is crucial for biomedical research, drug discovery, and diagnostics.
  • Existing methods for microparticle manipulation often face limitations in efficiency and complexity.

Purpose of the Study:

  • To develop a novel, highly efficient method for trapping microparticles in controlled arrays.
  • To demonstrate the application of trapped particles as microlenses for high-quality imaging.

Main Methods:

  • Real-time two-photon lithography in a controlled flow system.
  • Sequential stopping and flowing of liquid resin containing microparticles.
  • Designing custom trap structures for precise particle arrangement.

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Last Updated: Feb 17, 2026

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Main Results:

  • Achieved unprecedentedly high capture efficiency of ~100% on a one-bead-to-one-trap basis.
  • Successfully trapped polydisperse particles into desired arrays.
  • Demonstrated trapping of particle-cluster arrays with controlled particle numbers.
  • Utilized trapped particles as microlenses for high-quality imaging.

Conclusions:

  • The developed method offers a versatile platform for integrating bead-based assays.
  • This technology is a significant step towards innovative microfluidic, optofluidic, and single-cell analysis devices.
  • The system simplifies complexity by eliminating the need for persistent pressure after trapping.