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Updated: Feb 17, 2026

Pure Shift Nuclear Magnetic Resonance: a New Tool for Plant Metabolomics
Published on: July 31, 2021
Real-time homonuclear broadband decoupled pure shift COSY
Veera Mohana Rao Kakita1,2, Jagadeesh Bharatam1
1Centre for NMR and Structural Chemistry, CSIR-Indian Institute of Chemical Technology, Uppal Road, Tarnaka, Hyderabad, 500 007, India.
Abstract:
The present manuscript reports development and applications of real-time homonuclear broadband decoupled pure shift version of in-phase zero-quantum filtered COSY (PS-IPZF-COSY) and clean in-phase COSY (PS-CLIP-COSY) pulse schemes. In contrast to the conventional COSY schemes, these pure shift versions provide enhanced spectral resolution and simplify the chemical shift correlation analysis of scalar coupled spins in complex organic molecules, which are exemplified for erythromycin A, estradiol, and a mixture of estradiol and testosterone.
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