Related Experiment Video
Updated: Feb 17, 2026
Systems Analysis of the Neuroinflammatory and Hemodynamic Response to Traumatic Brain Injury
Published on: May 27, 2022
[The Role of CBS in Injury Time Estimation after Brain Contusion]
1Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China.
Objectives:
To observe the changes of cystathionine β-synthase （CBS） expression in the cerebral cortex after brain contusion at different times.
Methods:
An experimental model of traumatic brain injury （TBI） in mice was established by an improved weight-drop device. Then Western blotting and immunohistochemical examination were used to detect the CBS expression in cerebral cortex around injury at different time points （1 h, 6 h, 12 h, 1 d, 2 d, 3 d, 7 d）.
Results:
The results of Western blotting revealed that the expression level of CBS was down-regulated and reached its lowest level at the 3rd days after injury, and then restored to normal level after 7 days. The results of immunohistochemistry showed that CBS was present in the normal brain cortex. CBS expression gradually decreased at the 3rd days after injury, and then restored to normal level after 7 days.
Conclusions:
CBS has the potential to be a reference index for time estimation after brain contusion in forensic practice.
More Related Videos
06:50A Preclinical Controlled Cortical Impact Model for Traumatic Hemorrhage Contusion and Neuroinflammation
Published on: June 10, 2020
08:27Development of an Uncomplicated Mild Traumatic Brain Injury Model Modified by Weight-Drop Method and Evidenced by Magnetic Resonance Imaging
Published on: April 11, 2025