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[Individual Identification of Cartilage by Direct Amplification in Mass Disasters]
1Criminal Technology Division, Criminal Police Detachment, Shenzhen Public Security Bureau, Shenzhen 518040, China.
Objectives:
To explore the effectiveness of direct amplification for the STR analysis of cartilage, and to accelerate the effectiveness of disaster victim identification.
Methods:
Eighty-eight cartilage samples were directly amplified by PowerPle® 21 kit, and the results of genotyping were compared with that obtained by the magnetic beads method.
Results:
In 88 cartilage samples, the STR genotypes were successfully detected from 84 samples by direct amplification and magnetic beads method, and both the results of genotyping by two method were consistent.
Conclusions:
Direct amplification with PowerPlex® 21 kit can be used for STR genotyping of cartilages. This method is operated easily and promptly, which has a potential application in the individual identification of mass disasters.
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