Related Experiment Video
Updated: Feb 17, 2026

A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
[Determination of Sulfide Ion in Blood from Hydrogen Sulfide Poisoning Cases]
H S Qiang1,2, H Chen2, B H Shen2
1Pharmacy School of Wenzhou Medical University, Wenzhou 325035, China.
Objectives:
To establish a gas chromatography-mass spectrometry (GC-MS) method for the determination of sulfide ion in blood and apply it to the practical cases.
Methods:
The 1, 3, 5-tribromobenzene was selected as an internal standard, and 0.2 mL blood sample was collected and analyzed using GC-MS after α-Bromo-2, 3, 4, 5, 6-pentafluorobenzyl bromide derivatization.
Results:
The mass concentration of sulfide ion in blood had good linearity in the range of 0.2-40 μg/mL with a limit of detection (LOD) of 0.05 μg/mL. The mass concentration of sulfide ion was less than 0.05 μg/mL in blank blood from different sources such as healthy subjects and dead cases. In 3 sulfide poisoning cases, sulfide ion was detected in the blood samples of 6 victims, and the mass concentration range was 1.02-3.13 μg/mL.
Conclusions:
This study establishes a method for investigation of sulfide ion in blood which has been applied successfully to the cases of fatal sulfide poisonings.
Related Concept Videos
Precipitation and Co-precipitation
Determining the pH of Salt Solutions
Formation of Complex Ions
Preparation and Reactions of Sulfides
Amines to Sulfonamides: The Hinsberg Test
Generally, a primary amine reacts with the Hinsberg reagent to produce an N-substituted benzenesulfonamide. The electron-withdrawing sulfonyl...
Electrodeposition
Electrodeposition can...

