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Updated: Feb 17, 2026

Assaying Proteasomal Degradation in a Cell-free System in Plants
Published on: March 26, 2014
The role of endomembrane-localized VHA-c in plant growth
Aimin Zhou1,2, Tetsuo Takano3, Shenkui Liu4
1a Department of Landscape Architecture, College of Horticulture and Landscape Architecture , Northeast Agricultural University , Harbin , China.
Abstract:
In plant cells, the vacuolar-type H+-ATPase (V-ATPase), a large multis`ubunit endomembrane proton pump, plays an important role in acidification of subcellular organelles, pH and ion homeostasis, and endocytic and secretory trafficking. V-ATPase subunit c (VHA-c) is essential for V-ATPase assembly, and is directly responsible for binding and transmembrane transport of protons. In previous studies, we identified a PutVHA-c gene from Puccinellia tenuiflora, and investigated its function in plant growth. Subcellular localization revealed that PutVHA-c is mainly localized in endosomal compartments. Overexpression of PutVHA-c enhanced V-ATPase activity and promoted plant growth in transgenic Arabidopsis. Furthermore, the activity of V-ATPase affected intracellular transport of the Golgi-derived endosomes. Our results showed that endomembrane localized-VHA-c contributes to plant growth by influencing V-ATPase-dependent endosomal trafficking. Here, we discuss these recent findings and speculate on the VHA-c mediated molecular mechanisms involved in plant growth, providing a better understanding of the functions of VHA-c and V-ATPase.
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