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Neural crest ablation does not alter pulmonary vein development in the chick embryo
M T Phillips1, K Waldo, M L Kirby
1Department of Anatomy, Medical College of Georgia, Augusta 30912-2000.
The Anatomical Record
|March 1, 1989
Summary
Cardiac neural crest cells are crucial for outflow tract development but do not significantly impact pulmonary vein morphology. Ablation studies show no link between cardiac neural crest and venous anomalies like cor triatriatum.
Area of Science:
- Developmental biology
- Embryology
- Cardiovascular science
Background:
- Cranial neural crest cells are vital for pharyngeal arch and aortic arch artery development.
- Cardiac neural crest cells are implicated in outflow tract septation and anomalies like persistent truncus arteriosus.
- The role of neural crest in pulmonary vein development remains largely uninvestigated.
Purpose of the Study:
- To investigate the effect of cardiac neural crest ablation on the morphological development of the pulmonary vein.
- To determine if cardiac neural crest alterations contribute to venous anomalies.
Main Methods:
- Pulmonary vein presence confirmed via histological sections and computer reconstructions in normal chick embryos.
- Cardiac neural crest ablation performed at early embryonic stages (8-10).
- Intravascular casts prepared from operated, sham-operated, and control embryos for morphological analysis.
Main Results:
- Experimental embryos with persistent truncus arteriosus showed no morphological differences in pulmonary veins compared to controls.
- Ablation of cardiac neural crest had minimal impact on pulmonary vein development.
Conclusions:
- Cardiac neural crest alterations do not appear to be a significant factor in the morphological development of pulmonary veins.
- Findings suggest cardiac neural crest is not involved in venous anomalies such as cor triatriatum or anomalous pulmonary venous return.