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Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
Molecular typing of methicillin-resistant Staphylococcus aureus: Comparison of PCR-based open reading frame typing,
Shinji Ogihara1, Ryoichi Saito2, Etsuko Sawabe3
1Department of Clinical Laboratory, Tokyo Medical and Dental University Hospital of Medicine, Tokyo, Japan.
Abstract:
The recently developed PCR-based open reading frame typing (POT) method is a useful molecular typing tool. Here, we evaluated the performance of POT for molecular typing of methicillin-resistant Staphylococcus aureus (MRSA) isolates and compared its performance to those of multilocus sequence typing (MLST) and Staphylococcus protein A gene typing (spa typing). Thirty-seven MRSA isolates were collected between July 2012 and May 2015. MLST, spa typing, and POT were performed, and their discriminatory powers were evaluated using Simpson's index analysis. The MRSA isolates were classified into 11, 18, and 33 types by MLST, spa typing, and POT, respectively. The predominant strains identified by MLST, spa typing, and POT were ST8 and ST764, t002, and 93-191-127, respectively. The discriminatory power of MLST, spa typing, and POT was 0.853, 0.875, and 0.992, respectively, indicating that POT had the highest discriminatory power. Moreover, the results of MLST and spa were available after 2 days, whereas that of POT was available in 5 h. Furthermore, POT is rapid and easy to perform and interpret. Therefore, POT is a superior molecular typing tool for monitoring nosocomial transmission of MRSA.
Insights
The PCR-based open reading frame typing (POT) method offers superior discriminatory power for methicillin-resistant Staphylococcus aureus (MRSA) typing compared to MLST and spa typing. This rapid and effective technique aids in monitoring hospital-acquired MRSA infections.
Area of Science:
- Microbiology
- Molecular Biology
- Infectious Diseases
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant threat in healthcare settings.
- Accurate molecular typing is crucial for tracking MRSA transmission and implementing effective control measures.
Purpose of the Study:
- To evaluate the performance of the PCR-based open reading frame typing (POT) method for MRSA molecular typing.
- To compare POT's discriminatory power and speed against multilocus sequence typing (MLST) and spa typing.
Main Methods:
- Thirty-seven MRSA isolates were analyzed using MLST, spa typing, and POT.
- Simpson's index was employed to assess the discriminatory power of each typing method.
Main Results:
- POT identified 33 distinct MRSA types, significantly more than MLST (11 types) and spa typing (18 types).
- POT demonstrated the highest discriminatory power (0.992), surpassing MLST (0.853) and spa typing (0.875).
- POT results were obtained in 5 hours, considerably faster than MLST and spa typing (2 days).
Conclusions:
- POT is a highly discriminatory and rapid molecular typing tool for MRSA.
- The POT method is superior for monitoring nosocomial MRSA transmission due to its speed and accuracy.
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