Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An AI-derived peptide PCa2 suppresses pancreatic cancer growth via modulation of the ErbB/PI3K/AKT/mTOR/MYC signaling axis.

Cell communication and signaling : CCS·2026
Same author

Discovery of Two Novel Scorpion Venom Peptides Activating TRPML2 to Impair ZIKV Internalization.

Toxins·2026
Same author

Postnatal Mouse Brain Region-Resolved Peptidomic Resource of Conditional Ndel1 Loss: Comparison of Acidic and Alcoholic Extraction Strategies.

Journal of neurochemistry·2025
Same author

Heparin-Binding Proteins in the Nanoparticle Corona Enhance Cellular Uptake through Glycocalyx Interactions.

ACS nano·2025
Same author

Virulence and Stress-Related Proteins Are Differentially Enriched and <i>N</i>-Terminally Acetylated in Extracellular Vesicles from Virulent <i>Paracoccidioides brasiliensis</i>.

Journal of fungi (Basel, Switzerland)·2025
Same author

Enhancing the Bothropic Antivenom through a Reverse Antivenomics Approach.

Journal of proteome research·2025
Same journal

How "Soft" Are Your Gas Mixtures? Effects of Modifier Gas Types on the Dissociation of Labile Ions in Differential Mobility Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

A Robotic Sample Handling Platform for Fully Automated Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Direct Analysis in Real-Time Tandem Mass Spectrometry for Rapid Screening of Thirty-one Plant Growth Regulator Residues in <i>Rehmannia glutinosa</i>.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Characterization of Alkane Oxidation Products in a Corona-Discharge Reactor Using Ammonia-Doped Ion Mobility-Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Integration of a Modified Synchrotron Radiation Photoionization Time-of-Flight Mass Spectrometer with a Residual Gas Analyzer for Complementary Detection of Catalytic Products with Different Ionization Energies.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Screen for Tissue-Specific Markers of Drug-Induced Phospholipidosis Using Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
See all related articles

Related Experiment Video

Updated: Feb 17, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

16.9K

Quantitative Peptidomics with Five-plex Reductive Methylation labels.

Alexandre K Tashima1, Lloyd D Fricker2,3

  • 1Department of Biochemistry, Escola Paulista de Medicina, Federal University of Sao Paulo, Sao Paulo, SP, 04023-901, Brazil. aktashima@unifesp.br.

Journal of the American Society for Mass Spectrometry
|December 14, 2017
PubMed
Summary
This summary is machine-generated.

Accurate peptide quantitation is improved using reductive methylation labeling by correcting for natural isotope interference. This method reveals significant peptide level changes, enhancing quantitative proteomics and peptidomics accuracy.

Keywords:
CyanoborohydrideFormaldehydeIsotopic labels

More Related Videos

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
08:00

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Published on: November 4, 2021

2.7K
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

3.0K

Related Experiment Videos

Last Updated: Feb 17, 2026

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
11:53

Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling

Published on: July 1, 2014

16.9K
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
08:00

Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies

Published on: November 4, 2021

2.7K
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
09:40

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

Published on: April 28, 2022

3.0K

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • Quantitative proteomics and peptidomics utilize chemical tags with stable isotopes for relative peptide quantitation.
  • Reductive methylation with formaldehyde and sodium cyanoborohydride is a simple labeling method for amine modification.
  • A challenge in this method is the overlap from natural isotope abundance, complicating accurate quantitation.

Purpose of the Study:

  • To develop and validate equations for correcting natural isotope contributions in reductive methylation labeling.
  • To enable more accurate quantitation of peptide levels in complex biological samples.

Main Methods:

  • Calculated natural isotope contributions for 26 native peptides.
  • Derived equations to correct peak intensities based on these calculations.
  • Applied correction equations to quantitative data from vinblastine-treated HEK293T cells.

Main Results:

  • Developed and applied novel correction equations for reductive methylation labeling.
  • Demonstrated that the correction equations normalize replicate data to expected ratios.
  • Identified significant decreases in 21 peptides after vinblastine treatment in HEK293T cells.

Conclusions:

  • The derived equations enable accurate quantitation of small changes in peptide levels using reductive methylation.
  • This approach significantly improves the reliability of quantitative peptidomics and proteomics data.
  • The method is crucial for identifying subtle molecular changes in response to stimuli.