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Updated: Feb 17, 2026

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Calcium dynamics in tomato pollen tubes using the Yellow Cameleon 3.6 sensor.

María Laura Barberini1, Lorena Sigaut2, Weijie Huang3,4

  • 1Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, "Dr. Héctor Torres" (INGEBI-CONICET), Vuelta de Obligado 2490, C1428ADN, Buenos Aires, Argentina.

Plant Reproduction
|December 14, 2017
PubMed

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Summary

Tomato pollen tubes exhibit a dynamic cytoplasmic calcium gradient crucial for growth. Inhibiting calcium pumps or release disrupts this gradient, halting pollen tube elongation, highlighting calcium

Area of Science:

  • Plant reproductive biology
  • Cellular signaling
  • Molecular physiology

Background:

  • Pollen tube growth is essential for plant fertilization.
  • Cytoplasmic calcium concentration ([Ca2+]cyt) gradients are vital for pollen tube elongation.
  • The precise mechanisms generating and maintaining these calcium gradients remain unclear.

Purpose of the Study:

  • To investigate the role of calcium dynamics in tomato (Solanum lycopersicum) pollen tube growth.
  • To elucidate the mechanisms responsible for generating and maintaining the tip-focused [Ca2+]cyt gradient.
  • To assess the impact of pharmacological agents on calcium homeostasis and pollen tube development.

Main Methods:

  • Utilized transgenic tomato plants expressing the Yellow Cameleon 3.6 calcium sensor.
Keywords:
Calcium ATPasesCalcium concentration gradientIon channelsPollen tube growthSolanum lycopersicumYellow Cameleon 3.6

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  • Employed a pharmacological approach using cyclopiazonic acid (CPA) and 2-aminoethoxydiphenyl borate (2-APB).
  • Investigated the effects of GsMTx-4, a blocker of stretch-activated calcium channels.

    • Main Results:

    • Observed an oscillating, tip-focused [Ca2+]cyt gradient in tomato pollen tubes synchronized with growth.
    • CPA treatment disrupted subapical [Ca2+]cyt, abolished oscillations, and inhibited pollen tube growth.
    • 2-APB treatment eliminated tip [Ca2+]cyt, abolished the gradient, and arrested growth; GsMTx-4 inhibited germination and affected growth, suggesting calcium channel involvement.

    Conclusions:

    • The tip-focused [Ca2+]cyt gradient is essential for sustained tomato pollen tube growth.
    • Plant Ca2+-ATPases and endoplasmic reticulum calcium stores play critical roles in maintaining this gradient.
    • Stretch-activated calcium channels are implicated in regulating tomato pollen tube development and calcium dynamics.