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Updated: Feb 17, 2026

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Published on: March 5, 2013
Generation of Cell Lines Stably Expressing a Fluorescent Reporter of Nonsense-Mediated mRNA Decay Activity
Nadezhda M Markina1, Anton P Pereverzev1, Dmitry B Staroverov1
1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
Abstract:
Nonsense-mediated mRNA decay (NMD) is a mechanism of mRNA surveillance ubiquitous among eukaryotes. Importantly, NMD not only removes aberrant transcripts with premature stop codons, but also regulates expression of many normal genes. A recently introduced dual-color fluorescent protein-based reporter enables analysis of NMD activity in live cells. In this chapter we describe the method to generate stable transgenic cell lines expressing the splicing-dependent NMD reporter using consecutive steps of lentivirus transduction and Tol2 transposition.
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