Supercritical fluid chromatography: a promising alternative to current bioanalytical techniques

Area of Science:

• Analytical Chemistry
• Green Chemistry
• Separation Science

Background:

• The global environmental challenges have spurred the development of green chemistry principles.
• Supercritical Fluid Chromatography (SFC) has emerged as a key green analytical technique.
• Recent advancements in SFC instrumentation have revitalized its application due to enhanced performance.

Purpose of the Study:

• To detail the technical specifications of SFC relevant to bioanalysis.
• To provide a critical review of existing bioanalytical SFC methods.
• To highlight SFC's role in advancing green analytical chemistry.

Main Methods:

• Focus on the technical aspects and specifications of SFC for bioanalytical applications.
• Literature review of published bioanalytical SFC methods.
• Discussion of SFC's hyphenation capabilities, particularly with Mass Spectrometry (MS).

Main Results:

• SFC offers high efficiency, speed, and robustness, meeting bioanalysis demands.
• Its compatibility with MS facilitates sensitive and selective detection.
• The technique supports high-throughput screening and analysis.

Conclusions:

• Supercritical Fluid Chromatography is a highly efficient and fast technique suitable for modern bioanalysis.
• Its green chemistry attributes and MS hyphenation capabilities make it a valuable tool for high-throughput bioanalytical studies.
• SFC represents a significant advancement in separation science for environmental and biological sample analysis.