Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

8.7K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.7K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

8.8K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
8.8K
Caspases01:24

Caspases

14.2K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
14.2K
Regulation of the Unfolded Protein Response01:31

Regulation of the Unfolded Protein Response

3.1K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
3.1K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

10.1K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
10.1K
Apoptosis01:30

Apoptosis

15.4K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
15.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell evidence for PANoptosome complexes.

Nature reviews. Molecular cell biology·2026
Same author

Urolithin A activates aryl hydrocarbon receptor-NLRP6-mediated pathways in intestinal epithelial cells to modulate mucosal immunity and strengthen gut barrier integrity.

Nature communications·2026
Same author

Defining critical roles for ZBP1 in PANoptosis utilizing a novel genetic tool for disease modeling and therapeutic development.

bioRxiv : the preprint server for biology·2026
Same author

NLRP3 inflammasome promotes PAD2/PAD4 release and protein citrullination in rheumatoid arthritis.

Communications biology·2026
Same author

Preclinical characterization of AZD1163, a first-in-class anti-PAD2/4 bispecific antibody for the treatment of rheumatoid arthritis.

mAbs·2026
Same author

PANoptosis fuels the fire of arthritis.

Immunity·2026

Related Experiment Video

Updated: Feb 17, 2026

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
09:15

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Published on: October 20, 2022

2.8K

ZBP1: Innate Sensor Regulating Cell Death and Inflammation.

Teneema Kuriakose1, Thirumala-Devi Kanneganti1

  • 1Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN, USA.

Trends in Immunology
|December 14, 2017
PubMed
Summary

Z-DNA-binding protein 1 (ZBP1) acts as an innate sensor for viral infections, regulating cell death and inflammation. Its role in development and potential for lethality highlight its critical functions.

Keywords:
DAIRIPK1RIPK3ZBP1caspase-8cell deathinflammasomeinflammationinnate immunity

More Related Videos

Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.8K
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

11.3K

Related Experiment Videos

Last Updated: Feb 17, 2026

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells
09:15

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Published on: October 20, 2022

2.8K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.8K
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

11.3K

Area of Science:

  • Immunology
  • Molecular Biology
  • Cellular Biology

Background:

  • Z-DNA-binding protein 1 (ZBP1) is an interferon-inducible protein with nucleic acid and protein interaction domains.
  • Initially identified as a tumor-associated protein, ZBP1 is now recognized for its role in innate immunity.

Purpose of the Study:

  • To review the historical context and emerging roles of ZBP1.
  • To highlight ZBP1's function as a pathogen sensor and regulator of cell death and inflammation.
  • To identify knowledge gaps and future research directions for ZBP1.

Main Methods:

  • Literature review of studies on ZBP1.
  • Analysis of ZBP1's molecular domains (Z-DNA binding, RHIM).
  • Examination of ZBP1's involvement in viral sensing, cell death, and inflammation.

Main Results:

  • ZBP1 functions as a critical innate sensor for viral infections.
  • ZBP1 regulates key cellular processes including cell death, inflammasome activation, and inflammatory responses.
  • Dysregulation of ZBP1, particularly its RHIM-dependent interactions, can lead to developmental issues and perinatal lethality.

Conclusions:

  • ZBP1 is a central regulator of innate immunity, cell death, and inflammation.
  • Further research is needed to fully understand ZBP1 regulation and its diverse functions.
  • ZBP1 presents potential therapeutic targets for inflammatory diseases and viral infections.