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Updated: Feb 17, 2026

Identification of Antibacterial Immunity Proteins in Escherichia coli using MALDI-TOF-TOF-MS/MS and Top-Down Proteomic Analysis
Published on: May 23, 2021
First insights into a type II toxin-antitoxin system from the clinical isolate Mycobacterium sp. MHSD3, similar to
Daniel Jaén-Luchoro1,2,3, Francisco Aliaga-Lozano1,4, Rosa Maria Gomila5
1Microbiologia, Departament de Biologia, Universitat de les Illes Balears, Palma de Mallorca, Spain.
Abstract:
A putative type II toxin-antitoxin (TA) system was found in the clinical isolate Mycobacterium sp. MHSD3, a strain closely related to Mycobacterium chelonae. Further analyses of the protein sequences of the two genes revealed the presence of domains related to a TA system. BLAST analyses indicated the presence of closely related proteins in the genomes of other recently published M. chelonae strains. The functionality of both elements of the TA system was demonstrated when expressed in Escherichia coli cells, and the predicted structure of the toxin is very similar to those of well-known zeta-toxins, leading to the definition of a type II TA system similar to epsilon/zeta TA systems in strains that are closely related to M. chelonae.
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