Related Experiment Video
Updated: Feb 17, 2026
14:23
Recombineering Homologous Recombination Constructs in Drosophila
Published on: July 13, 2013
19.8K
RECOMBINATION CHAMBERS-DO THE OLD IDEAS REMAIN USEFUL?
1Institute of Metrology and Biomedical Engineering, Faculty of Mechatronics, Warsaw University of Technology, Sw. A. Boboli 8, Warsaw, Poland.
Radiation Protection Dosimetry
|December 14, 2017
Summary
Recombination chambers, developed alongside radiation quality factor concepts, offer practical applications for measuring dosimetric quantities in radiation protection.
Area of Science:
- Medical Physics
- Radiation Dosimetry
- Health Physics
Background:
- The concept of radiation quality factor, based on linear energy transfer, emerged concurrently with early recombination methods.
- Recombination chambers and associated techniques have evolved significantly with advancements in dosimetry and radiation protection principles.
Purpose of the Study:
- To present the key characteristics of recombination chambers.
- To explore the potential uses of recombination chambers in practical dosimetric measurements.
Main Methods:
- Review of historical development of recombination methods.
- Description of the fundamental principles of recombination chambers.
- Discussion of applications in radiation dosimetry.
Main Results:
- Recombination chambers have a long history linked to fundamental radiation concepts.
- These chambers have undergone extensive development over time.
- They possess viable applications in current dosimetric measurement systems.
Conclusions:
- Recombination chambers are a well-established technique in radiation dosimetry.
- Their features and applications remain relevant for practical radiation protection and measurement systems.
Related Concept Videos
02:53Conservative Site-specific Recombination and Phase Variation
6.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
6.9K
02:31Homologous Recombination
63.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
63.7K
02:31Homologous Recombination
6.8K
6.8K
01:30Crossing Over
6.7K
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I,...
6.7K
02:32Exon Recombination
4.2K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...
Exon shuffling follows “splice frame rules.” Each exon...
4.2K
02:08Gene Conversion
10.7K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
10.7K