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Application of the AMLprofiler Diagnostic Microarray in the South African Setting
S S Kappala1, M Alessandrini1, T Matlhako2
1Institute for Cellular and Molecular Medicine, Department of Immunology, Faculty of Health Sciences, and SAMRC Extramural Unit for Stem Cell Research and Therapy, University of Pretoria, Pretoria, South Africa.
Stem Cells International
|December 15, 2017
Summary
AMLprofiler, a diagnostic microarray, accurately identifies biomarkers for acute myeloid leukemia (AML). This method offers comparable results to standard diagnostics, improving patient risk stratification and potentially reducing diagnosis time.
Area of Science:
- Hematology
- Molecular Diagnostics
- Oncology
Background:
- Acute myeloid leukemia (AML) presents significant heterogeneity in treatment response and survival rates.
- Accurate diagnosis and prognostic stratification are crucial for effective AML management.
Purpose of the Study:
- To compare the diagnostic performance of AMLprofiler, an in vitro diagnostic microarray, against routine methodologies for acute myeloid leukemia in South Africa.
- To evaluate the consistency, cost-effectiveness, and time efficiency of AMLprofiler.
Main Methods:
- RNA was extracted from bone marrow and peripheral blood samples of de novo AML patients.
- Samples were processed using Affymetrix Gene Profiling Reagent kits for microarray analysis.
- AMLprofiler results were compared with standard diagnostic procedures.
Main Results:
- AMLprofiler demonstrated highly comparable results to routine AML diagnostic methods.
- The microarray identified additional relevant biomarkers, including CEBPA double mutants, NPM1 variants, and altered BAALC/EVI1 expression, not routinely tested.
- AMLprofiler enabled prognostic classification for 26% of AML patients, including those with no positive biomarker identified by standard methods.
Conclusions:
- AMLprofiler is a comprehensive diagnostic tool for acute myeloid leukemia, offering comparable accuracy to standard methods.
- The microarray enhances risk stratification and potentially decreases time to result for AML diagnosis.
- Incorporating AMLprofiler can improve the diagnostic capabilities for AML patients in South Africa.
