Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reversible and irreversible rotating field-induced membrane modifications.

G Fuhr1, T Müller, R Hagedorn

  • 1Department of Biology, Humboldt University of Berlin, G.D.R.

Biochimica Et Biophysica Acta
|March 27, 1989
PubMed
Summary

Rotating electric fields induce phase-shifted transmembrane potentials, affecting cell behavior. High field strengths cause irreversible changes and membrane rupture, with characteristic frequency and angular velocity describing non-linear electrorotation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Behavior of cells in rotating electric fields with account to surface charges and cell structures.

Biophysical journal·2009
Same author

Evidence from numerical simulations of transport-barrier relaxations in tokamak edge plasmas in the presence of electromagnetic fluctuations.

Physical review letters·2008
Same author

Ultrasonic standing wave manipulation technology integrated into a dielectrophoretic chip.

Lab on a chip·2007
Same author

Malaria early warnings based on seasonal climate forecasts from multi-model ensembles.

Nature·2006
Same author

Probabilistic prediction of climate using multi-model ensembles: from basics to applications.

Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2006
Same author

A novel class of amitogenic alginate microcapsules for long-term immunoisolated transplantation.

Annals of the New York Academy of Sciences·2002

Area of Science:

  • Biophysics
  • Electrical Engineering
  • Cell Biology

Background:

  • Transmembrane potentials are crucial for cell function.
  • Electric fields can alter cell membrane properties.
  • Understanding cell responses to rotating electric fields is important for various applications.

Purpose of the Study:

  • To analyze charge distribution induced by transmembrane potentials in rotating electric fields.
  • To investigate the frequency-dependent phase shifts and electrical property influences.
  • To explore non-linear electrorotation effects, including reversible and irreversible changes, and membrane rupture.

Main Methods:

  • Analysis of charge distribution under induced transmembrane potentials.
  • Investigation of phase shifts in rotating electric fields (d.c. and a.c. fields as comparison).

Related Experiment Videos

  • Extension of study to non-linear field strength ranges for electrorotation.

    • Main Results:

    • Induced peak potential differences are phase-shifted in rotating fields, differing from d.c. and a.c. fields.
    • Phase differences are frequency-dependent and influenced by cell and medium electrical properties.
    • Non-linear electrorotation exhibits reversible and irreversible changes, characterized by fc1 and ωc, ultimately leading to membrane rupture at high field strengths.

    Conclusions:

    • Rotating electric fields induce complex, frequency-dependent transmembrane potential shifts.
    • Non-linear electrorotation reveals critical thresholds for cellular changes and membrane integrity.
    • Characteristic frequency and angular velocity are key parameters for describing non-linear electrorotation behavior.