Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Focusing of Light in the Eye01:16

Focusing of Light in the Eye

6.4K
Light rays enter the eye through the cornea, a transparent dome-shaped tissue that is the eye's outermost layer. The cornea bends or refracts, light rays traveling to the pupil. The shape of the cornea determines how much of the light is bent and whether the image will be focused correctly on the retina at the back of the eye. Once the light has passed through both refraction layers, it converges into a single focal point onto a small area. This is where photoreceptors start transforming...
6.4K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

14.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
14.5K
Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

11.2K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
11.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantumness of hybrid systems under quantum noise.

Scientific reports·2026
Same author

Managing Blood Pressure in Dialysis Patients: A Summary of UK Clinical Practice Guideline.

Kidney international reports·2026
Same author

Balancing Efficiency and Accuracy in Hepatitis C Rapid Antibody Testing: Insights From a Cluster Randomised Crossover Trial.

Journal of viral hepatitis·2025
Same author

The performance of small sample correction methods for controlling type I error when analyzing parallel cluster randomized trials: a systematic review of simulation studies.

Journal of clinical epidemiology·2025
Same author

TIAMAT- towards an interdisciplinary automated malnutrition screening tool.

Clinical nutrition ESPEN·2025
Same author

Standardising and simplifying the Global Leadership Initiative on Malnutrition (GLIM) for its more general application.

Clinical nutrition ESPEN·2024
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Feb 16, 2026

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.7K

Focusing anomalies with binary diffractive optical elements.

O Bouzid, S Haddadi, M Fromager

    Applied Optics
    |December 15, 2017
    PubMed
    Summary
    This summary is machine-generated.

    A simple binary diffractive optic can unexpectedly intensify a focused laser beam. This study explains these focusing anomalies and suggests applications for super-resolution microscopy.

    More Related Videos

    Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
    09:43

    Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

    Published on: March 20, 2017

    10.4K
    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    10.4K

    Related Experiment Videos

    Last Updated: Feb 16, 2026

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.7K
    Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping
    09:43

    Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping

    Published on: March 20, 2017

    10.4K
    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator
    08:39

    Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

    Published on: January 28, 2019

    10.4K

    Area of Science:

    • Optics and Photonics
    • Microscopy

    Background:

    • Binary diffractive optics are established tools for laser beam shaping.
    • Existing implementations include etched optics and spatial light modulators.

    Purpose of the Study:

    • To demonstrate a simple, one-step binary optic for laser beam manipulation.
    • To investigate counterintuitive focusing anomalies and their underlying optical aberrations.
    • To explore potential applications in super-resolution microscopy.

    Main Methods:

    • Fabrication and experimental testing of a one-step binary diffractive optic.
    • Analysis of optical aberrations present in the binary optic.
    • Theoretical explanation of observed focusing anomalies.

    Main Results:

    • The binary optic successfully enhanced the intensity of a focused laser beam.
    • Observed counterintuitive focusing anomalies were documented.
    • Optical aberrations were identified as the cause of these anomalies.

    Conclusions:

    • A simple binary optic can achieve enhanced beam intensity and exhibit focusing anomalies.
    • Understanding these aberrations is key to optimizing diffractive optics.
    • This work provides a foundation for improved refractive/diffractive optical systems for super-resolution microscopy.