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Optimized Bone Sampling Protocols for the Retrieval of Ancient DNA from Archaeological Remains
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A Simple and Efficient Method of Extracting DNA from Aged Bones and Teeth.
Qiqi Liu1, Liyan Liu1, Minli Zhang1
1Beijing Institute of Radiation Medicine, Beijing, 100850, China.
Journal of Forensic Sciences
|December 15, 2017
Summary
A new DNA extraction method (OM) efficiently retrieves more STR loci from ancient bones and teeth compared to commercial kits. This cost-effective technique improves DNA analysis for forensic and anthropological studies.
Area of Science:
- Forensic Science
- Molecular Biology
- Anthropology
Background:
- Extracting DNA from aged bones and teeth is challenging due to low DNA quantity and high degradation.
- Existing commercial DNA extraction kits may not be optimal for ancient samples.
Purpose of the Study:
- To develop a simple, efficient, and cost-effective DNA extraction method for aged bones and teeth.
- To enhance the detection rate of Short Tandem Repeat (STR) typing from challenging forensic samples.
Main Methods:
- A novel DNA extraction method (OM) was developed and applied to 20 aged bone and teeth samples (approx. 60 years old).
- DNA yield and STR typing profiles were compared between the OM method and a commercial kit (PrepFiler™ BTA Forensic DNA Extraction Kit - BM).
Main Results:
- The OM method yielded comparable total DNA concentrations to the BM method (p > 0.05).
- Significantly higher numbers of STR loci were detected using the OM method compared to the BM method (p < 0.05).
Conclusions:
- The developed OM method is effective for DNA extraction from aged bones and teeth.
- This method offers improved STR typing success rates and potential cost reductions compared to commercial alternatives.
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