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The Future of Multiplexed Eukaryotic Genome Engineering.

David B Thompson^1,2, Soufiane Aboulhouda^1,2, Eriona Hysolli^1,2

¹Department of Genetics, Harvard Medical School , Boston, Massachusetts, United States.

ACS Chemical Biology

|December 15, 2017

Summary

Multiplex genome editing enables simultaneous modifications in large eukaryotic genomes. This technology holds promise for advanced biomedical research, genetic engineering, and novel therapeutics.

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Area of Science:

Genomics and Molecular Biology
Biotechnology and Genetic Engineering

Background:

Multiplex genome editing involves introducing multiple genetic modifications simultaneously.
This technology is crucial for advancing biological engineering and biomedical research.

Purpose of the Study:

To review the current state and limitations of multiplex genome editing in large eukaryotic genomes.
To explore future applications and technological advancements in multiplex genome editing.

Main Methods:

Focuses on a perspective review of multiplex genome editing technologies.
Discusses CRISPR-based technologies, intracellular delivery, and DNA assembly approaches.

Main Results:

Highlights the current capabilities and limitations of multiplex genome editing.
Identifies potential applications in therapeutics, industrial uses, genome writing, and de-extinction.

Conclusions:

Multiplex genome editing is a rapidly developing field with transformative potential.
Future advancements in CRISPR, delivery, and assembly will enable large-scale genome engineering.