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Antibody separation using lectin modified poly(HEMA-EDMA) hydrogel membranes.

Esra Feyzioğlu Demir1, Cansu Ilke Kuru2, Murat Uygun3,4

  • 1a Medical Laboratory Technique , Vocational School of Health Services, Izmir University of Economics , Izmir , Turkey.

Journal of Biomaterials Science. Polymer Edition
|December 16, 2017
PubMed
Summary

This study developed Concanavalin A-functionalized hydrogel membranes for antibody separation. These membranes demonstrate high IgG adsorption capacity and excellent reusability for effective purification from aqueous solutions.

Keywords:
IgG adsorptionLectin affinity chromatographyconcanavalin Ahydrogel membranessilanization

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Area of Science:

  • Biomaterials Science
  • Separation Science
  • Polymer Chemistry

Background:

  • Antibody purification is crucial for therapeutic applications.
  • Developing efficient and reusable separation materials is essential.
  • Hydrogel membranes offer tunable properties for biomolecule separation.

Purpose of the Study:

  • To synthesize and characterize Concanavalin A-poly(2-hydroxyethyl methacrylate-ethylene dimethacrylate) hydrogel membranes.
  • To evaluate the membranes' capacity for antibody (IgG) adsorption and elution.
  • To assess the reusability and stability of the hydrogel membranes for IgG separation.

Main Methods:

  • Photopolymerization technique for hydrogel membrane synthesis.
  • Characterization using Scanning Electron Microscopy, FTIR, Elemental Analysis, and swelling tests.
  • Adsorption-elution studies to determine IgG binding capacity and optimal conditions.
  • Reusability testing over multiple adsorption-desorption cycles.

Main Results:

  • Synthesized membranes exhibited rough morphology and spherical structures.
  • Concanavalin A binding capacity was 3.52 mg/g.
  • Maximum IgG adsorption capacity reached 26.81 mg/g.
  • Successful elution of adsorbed IgG using 2.0 M NaCl.
  • No significant loss in adsorption capacity after five reuse cycles.

Conclusions:

  • Concanavalin A-functionalized hydrogel membranes are effective for IgG adsorption.
  • The developed membranes show high adsorption capacity, stability, and reusability.
  • These hydrogel membranes show promise as efficient sorbents for antibody purification.