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Updated: Feb 16, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
Adipose KLF15 Controls Lipid Handling to Adapt to Nutrient Availability
Keiichiro Matoba1, Yuan Lu2, Rongli Zhang2
1Case Cardiovascular Research Institute, Department of Medicine, Case Western Reserve University School of Medicine, Harrington Heart and Vascular Institute, University Hospitals Cleveland Medical Center, Cleveland, OH 44106, USA; Division of Diabetes, Metabolism and Endocrinology, Department of Internal Medicine, Jikei University School of Medicine, Tokyo 105-8461, Japan.
Kruppel-like factor 15 (KLF15) in fat cells controls energy storage. Mice lacking KLF15 in adipose tissue are protected from obesity and show altered energy balance during fasting.
Area of Science:
- Metabolic regulation
- Adipocyte biology
- Transcriptional control
Background:
- Adipose tissue triglyceride metabolism is vital for energy homeostasis.
- Dysregulation contributes to metabolic diseases like obesity.
- Hormonal regulation is known, but transcriptional control remains unclear.
Purpose of the Study:
- To investigate the role of transcription factor KLF15 in adipocyte lipid metabolism.
- To elucidate the impact of adipose KLF15 on systemic energy balance.
Main Methods:
- Generation of adipose-specific KLF15 knockout (AK15KO) mice.
- Analysis of adiposity, lipid metabolism, and gene expression.
- Assessment of systemic energetics during fed and fasted states.
Main Results:
- AK15KO mice exhibit reduced adiposity and protection from diet-induced obesity.
- Adipose KLF15 promotes triglyceride synthesis and inhibits lipolysis.
- AK15KO mice show enhanced lipolysis and altered systemic energetics during fasting.
Conclusions:
- Adipose KLF15 is a critical regulator of adipocyte lipid turnover.
- KLF15 influences insulin-dependent lipid storage.
- KLF15 plays a significant role in systemic energy balance.
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