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Updated: Feb 16, 2026

Preparation of Quality Inositol Pyrophosphates
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Prion propagation and inositol polyphosphates.

Reed B Wickner1, Herman K Edskes2, Evgeny E Bezsonov2

  • 1Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, 20892-0830, USA. wickner@helix.nih.gov.

Current Genetics
|December 16, 2017
PubMed
Summary
This summary is machine-generated.

Certain inositol polyphosphates (IPs) are crucial for the propagation of the [PSI+] prion, a protein aggregate. Specific IPs support prion formation, while others inhibit it, impacting cellular processes like translation.

Keywords:
Inositol polyphosphatePrionSiw14Sup35[PSI+]

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High-throughput Screening for Protein-based Inheritance in S. cerevisiae
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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Prion Biology

Background:

  • The [PSI+] prion is an amyloid form of the Sup35 protein, essential for translation termination.
  • Prion propagation is influenced by cellular factors, and understanding these is key to controlling prion diseases.

Purpose of the Study:

  • To identify cellular factors that regulate the propagation of the [PSI+] prion.
  • To investigate the role of inositol polyphosphates (IPs) in prion formation and maintenance.

Main Methods:

  • Searched for anti-prion systems affecting [PSI+] prion propagation.
  • Investigated the effects of various soluble inositol polyphosphates (IPs) on prion stability and formation.

Main Results:

  • Identified specific soluble inositol polyphosphates (IPs) as critical for [PSI+] prion propagation.
  • Found that 5-diphosphoinositol tetra(or penta)kisphosphate and inositol hexakisphosphate support prion propagation.
  • Observed that 1-diphosphoinositol pentakisphosphate inhibits [PSI+] prion propagation.

Conclusions:

  • Soluble inositol polyphosphates (IPs) are important regulators of [PSI+] prion propagation.
  • These IPs influence diverse cellular processes, including mRNA export, telomere length, and metabolism.
  • Targeting specific IPs could offer a strategy for controlling prion formation and related diseases.