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Updated: Feb 16, 2026
Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
Published on: October 10, 2013
Assessing a multiplex-targeted proteomics approach for the clinical diagnosis of periodontitis using saliva samples
Brenda Mertens1, Valérie Orti1, Jérôme Vialaret2
1U.F.R. d'Odontologie, Département de Parodontologie, 545, avenue du Professeur Jean-Louis Viala, 34 193 Montpellier, Cedex 5.
Aim:
The present study focused on the research of new biomarkers based on the liquid chromatography-multiple-reaction monitoring (MRM) proteomic profile in whole saliva of patients with periodontitis compared with periodontal healthy patients.
Methods:
A 30-min multiplexed liquid chromatography-MRM method was used for absolute quantification of 35 plasma biomarkers in saliva from control patients and patients with periodontitis.
Results:
Three proteins namely hemopexin, plasminogen and α-fibrinogen were shown to be clearly related to the presence of periodontitis compared with healthy patients. Apolipoprotein H was found to discriminate for the first time chronic and aggressive periodontitis.
Conclusion:
Our results indicate that this innovative MRM method could be used to screen for periodontitis in clinical environment. Furthermore, apolipoprotein H was found to be a discriminant biomarker of aggressive periodontitis.
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