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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins
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Encapsulation challenges, the substantial issue in solid lipid nanoparticles characterization.

Sara Daneshmand1, Shiva Golmohammadzadeh2, Mahmoud R Jaafari3

  • 1Department of Pharmaceutics, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Journal of Cellular Biochemistry
|December 16, 2017
PubMed
Summary
This summary is machine-generated.

Solid lipid nanoparticles (SLNs) are crucial for drug delivery. This review details methods for measuring entrapment efficiency (EE) in SLNs, highlighting factors affecting accuracy and discussing pros and cons.

Keywords:
drug loadingdrug solubilityentrapment efficiencyparticle sizesolid lipid nanoparticles

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Area of Science:

  • Pharmaceutical Sciences
  • Nanotechnology
  • Drug Delivery

Background:

  • Solid lipid nanoparticles (SLNs) are colloidal carriers for sustained release of lipophilic drugs.
  • Entrapment efficiency (EE) is a key parameter for characterizing SLNs and estimating drug loading capacity.
  • Accurate EE measurement is critical but challenging due to various influencing factors.

Purpose of the Study:

  • To review and introduce methods for determining entrapment efficiency (EE) in solid lipid nanoparticles (SLNs).
  • To discuss the advantages and disadvantages of different EE measurement techniques.
  • To highlight factors influencing EE determination in SLN formulations.

Main Methods:

  • Direct method: Directly measuring the encapsulated drug within SLNs.
  • Indirect method: Measuring the unencapsulated drug in the supernatant after SLN separation.
  • Discussion of analytical challenges in drug quantification for EE calculation.

Main Results:

  • EE measurement is influenced by stirring speed/duration, surfactant/emulsifier concentration, and lipid type/concentration.
  • Both direct and indirect methods have specific challenges related to drug analysis accuracy.
  • No single method is universally superior; choice depends on the specific SLN system and drug.

Conclusions:

  • Accurate entrapment efficiency (EE) determination is vital for solid lipid nanoparticles (SLNs) drug loading.
  • Understanding the variables affecting EE and the limitations of measurement methods is crucial.
  • This review provides insights into selecting appropriate EE determination strategies for SLN research and development.