Related Experiment Video
Updated: Feb 16, 2026
14:16
Fluorescence detection methods for microfluidic droplet platforms
Published on: December 10, 2011
22.9K
Fluorescence goes from strength to strength in MAF
David J S Birch1, Yves Mely2, Otto Wolfbeis3
1The Photophysics Research Group, Centre for Molecular Nanometrology, University of Strathclyde, Department of Physics, SUPA, John Anderson Building, 107 Rottenrow, Glasgow, G4 0NG, United Kingdom.
Methods and Applications in Fluorescence
|December 16, 2017
Summary
Methods and Applications in Fluorescence (MAF) achieved a 10% impact factor increase to 2.7. All articles are now discoverable on PubMed® following inclusion by MEDLINE.
Area of Science:
- Scientific Publishing
- Bibliometrics
- Journal Metrics
Background:
- The journal Methods and Applications in Fluorescence (MAF) has seen significant growth.
- Indexing in major databases is crucial for scientific journal visibility.
More Related Videos
Related Concept Videos
01:29Atomic Fluorescence Spectroscopy
994
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
994
01:12Immunofluorescence Microscopy
13.9K
A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
13.9K
01:25Fluorescence and Phosphorescence: Instrumentation
1.6K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.6K
01:37Super-resolution Fluorescence Microscopy
14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
01:19Protein Dynamics in Living Cells
2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
01:29Two-Dimensional Microscopy in Microbiology
1.7K
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
1.7K