Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complementation Tests00:49

Complementation Tests

6.3K
A complementation test is a simple cross to identify whether the two mutations are located on the same gene or different genes. It was first performed by Edward Lewis in the 1940s while working on fruit flies. He developed the test to identify the location and arrangement of different mutations on chromosomes.
Organisms heterozygous for different mutations are crossed pairwise in all combinations. If present on different genes, the mutations can complement each other by providing the missing...
6.3K
Mismatch Repair01:20

Mismatch Repair

6.7K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
6.7K
Complementary DNA01:44

Complementary DNA

31.8K
Overview
31.8K
Point and Frameshift Mutations01:30

Point and Frameshift Mutations

1.3K
Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...
1.3K
Mutations in Microorganisms01:18

Mutations in Microorganisms

793
Mutations are heritable changes in an organism’s genome involving alterations in the base sequence of DNA or RNA. These changes can influence cellular processes and phenotypic traits, potentially transforming the unaltered wild type into a mutant form. Such changes, termed forward mutations, are pivotal in shaping the genetic diversity of organisms.RNA viruses exhibit the highest mutation rates due to the absence of robust proofreading mechanisms during genome replication. In contrast,...
793

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Can habitat modification in the native range promote invasion?

Trends in ecology & evolution·2026
Same author

Using Landscape Genomics to Define Species Distributions, Delineate Seed Zones, and Predict Genomic Offset to Future Climate for the Interior Spruce Hybrid Complex (Picea glauca, Picea engelmannii, and Their Hybrids).

Global change biology·2026
Same author

Genomic ancestry predicts rapid responses to drought across spatiotemporal scales.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The genomic basis of adaptive leaf variation in the Galápagos giant daisies.

Nature communications·2026
Same author

Rapid evolution predicts demographic recovery after extreme drought.

Science (New York, N.Y.)·2026
Same author

Sunflower genetics and genomics: from fundamental evolutionary insights to crop improvement.

Trends in genetics : TIG·2026

Related Experiment Video

Updated: Feb 16, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

8.6K

Bioinformatically predicted deleterious mutations reveal complementation in the interior spruce hybrid complex.

Gina L Conte1,2, Kathryn A Hodgins3,4, Sam Yeaman3,5

  • 1Department of Forest and Conservation Sciences, University of British Columbia, 3041-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada. conte@zoology.ubc.ca.

BMC Genomics
|December 17, 2017
PubMed
Summary

Hybrid interior spruce (Picea glauca x P. engelmannii) show reduced mutation load due to complementation of deleterious alleles. This genetic mechanism may enhance hybrid fitness and adaptation.

Keywords:
ComplementationConifersDeleterious mutationsHybridizationMutation loadPopulation genomicsSpruce

More Related Videos

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

7.8K
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
11:42

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

15.1K

Related Experiment Videos

Last Updated: Feb 16, 2026

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
07:55

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

8.6K
Identifying Mutations by High Resolution Melting in a TILLING Population of Rice
06:10

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

7.8K
Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions
11:42

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

Published on: September 30, 2016

15.1K

Area of Science:

  • Evolutionary genetics
  • Plant genomics
  • Population genetics

Background:

  • Mutation load is theoretically reduced in hybrids through the complementation of deleterious alleles.
  • Local adaptation in hybrids can complicate direct phenotypic assessments of reduced mutation load.
  • Analyzing the distribution of putatively deleterious alleles offers an alternative method to assess variation in mutation load.

Purpose of the Study:

  • To investigate mutation load in the interior spruce (Picea glauca x P. engelmannii) hybrid complex.
  • To assess the distribution of putatively deleterious alleles in relation to hybrid local adaptation.
  • To determine if hybrids exhibit reduced mutation load compared to parental species.

Main Methods:

  • Utilized PROVEAN for bioinformatic prediction of deleterious non-synonymous alleles based on conservation and amino acid changes.
  • Analyzed allele frequencies and homozygosity patterns of predicted deleterious alleles in white spruce, Engelmann spruce, and their hybrids.
  • Assessed phenotypic effects on juvenile growth rate, although none were detected for rare deleterious alleles.

Main Results:

  • Predicted deleterious alleles occurred at lower average frequencies than non-deleterious alleles.
  • Engelmann spruce exhibited higher proportions of deleterious alleles and homozygous deleterious loci than white spruce.
  • Hybrids displayed intermediate proportions of deleterious alleles but the lowest proportion of homozygous deleterious loci compared to parental species.

Conclusions:

  • Reduced homozygosity for deleterious alleles in hybrids suggests a lower mutation load due to complementation.
  • This complementation of deleterious alleles likely contributes to enhanced hybrid fitness.
  • The findings support the hypothesis that genetic mechanisms, beyond local adaptation, contribute to hybrid vigor in interior spruce.