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Updated: Feb 16, 2026

Conditional Reprogramming of Pediatric Human Esophageal Epithelial Cells for Use in Tissue Engineering and Disease Investigation
Published on: March 22, 2017
Development of one control and one tumor-specific induced pluripotent stem cell line from laryngeal carcinoma patient
Yamin Zhang1, Jing Zhu2, Yaozhang Dai1
1The Otorhinolaryngology-Head and Neck Surgery Hospital, The 1st Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Abstract:
Skin fibroblasts and tumor fibroblasts were extracted from a 64-year old male patient clinically diagnosed with laryngeal carcinoma. Control and tumor specific induced pluripotent stem cells were reprogrammed with 5 reprogramming factors, Klf-4, c-Myc, Oct-4, Sox-2, and Lin-28, using the messenger RNA reprogramming system. The transgene-free iPSC lines showed pluripotency, confirmed by immunofluorescence staining. The iPSC lines also showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This in vitro cellular model can be used to study the oncogenesis and pathogenesis of laryngeal carcinoma.
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