Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specific oligodeoxynucleotide probes obtained through RNA sequencing.

K Köhrer1, T M Kutchan, H Domdey

  • 1Laboratorium für Molekulare Biologie-Genzentrum-der Ludwig-Maximilians-Universität München, Martinsried, FRG.

DNA (Mary Ann Liebert, Inc.)
|March 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multicenter validation of cancer gene panel-based next-generation sequencing for translational research and molecular diagnostics.

Virchows Archiv : an international journal of pathology·2018
Same author

Intrahepatic cholestasis of pregnancy (ICP): case report and review of the literature.

Zeitschrift fur Gastroenterologie·2016
Same author

Synthetic gestagens exert differential effects on arterial thrombosis and aortic gene expression in ovariectomized apolipoprotein E-deficient mice.

British journal of pharmacology·2014
Same author

Cytodifferentiation and alkaloid accumulation in cultured cells of Papaver bracteatum.

Plant cell reports·2013
Same author

Correlation of the appearance of morphinan alkaloids and laticifer cells in germinating Papaver bracteatum seedlings.

Plant cell reports·2013
Same author

Implication of tyramine in the biosynthesis of morphinan alkaloids in Papaver.

Planta·2013

This study presents a two-step method to test oligodeoxynucleotide probe specificity for RNA targets. It enables designing highly specific probes, avoiding lengthy sequencing of cross-hybridizing fragments.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Oligodeoxynucleotide probes are crucial for molecular diagnostics and research.
  • Ensuring probe specificity is vital to avoid erroneous results.
  • Current methods for probe validation can be time-consuming and inefficient.

Purpose of the Study:

  • To develop a rapid and reliable method for assessing the specificity of mixed oligodeoxynucleotide hybridization probes.
  • To provide a basis for designing long, non-degenerate, and highly specific probes.
  • To circumvent laborious subcloning and sequencing of cross-hybridizing fragments.

Main Methods:

  • Utilized mixed oligodeoxynucleotide probes derived from known peptide sequences.
  • Employed primer extension reactions with poly(A)+RNA templates, dNTPs, and ddNTPs to generate cDNA.

Related Experiment Videos

  • Compared cDNA transcript lengths to predicted sequences to determine probe hybridization specificity.
  • Performed indirect RNA sequence analysis using validated probes.

    • Main Results:

    • Successfully developed a two-step procedure to evaluate probe specificity.
    • Demonstrated that cDNA transcript length analysis accurately predicts hybridization.
    • Confirmed probe specificity through indirect RNA sequencing.
    • Generated data to guide the design of superior, specific probes.

    Conclusions:

    • The developed method efficiently tests oligodeoxynucleotide probe specificity.
    • This approach significantly streamlines the design of specific probes for RNA targets.
    • Offers a faster alternative to traditional methods for probe validation and gene identification.