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Expression of miRNA 155, FOXP3 and ROR gamma, in children with moderate and severe atopic dermatitis

Massimiliano Bergallo1,2, Martina Accorinti1, Ilaria Galliano1,2

  • 1Department of Pediatrics, Azienda Ospedaliera Universitaria Città della Salute e della Scienza di Torino, Turin, Italy.

Giornale Italiano Di Dermatologia E Venereologia : Organo Ufficiale, Societa Italiana Di Dermatologia E Sifilografia
|December 19, 2017
PubMed
Abstract

Insights

This study found higher levels of microRNA 155 (miRNA 155) and FOXP3 in children with atopic dermatitis. These findings suggest a link between miRNA 155, T helper 17 (Th17) cells, and regulatory T (Treg) cells in atopic dermatitis pathogenesis.

Area of Science:

  • Immunology
  • Dermatology
  • Molecular Biology

Background:

  • Atopic dermatitis involves chronic skin inflammation, with its association with microRNA 155 (miRNA 155) not fully understood.
  • Investigating miRNA 155, T helper type 17 (Th17) cells, and regulatory T (Treg) cells is crucial for understanding atopic dermatitis.

Purpose of the Study:

  • To evaluate the expression of miRNA 155 in children diagnosed with atopic dermatitis.
  • To assess the levels of Th17 and Treg cells in pediatric patients with atopic dermatitis.
  • To explore the relationship between miRNA 155 and immune cell populations in atopic dermatitis.

Main Methods:

  • The study included 23 children with atopic dermatitis and 23 healthy controls.
  • Real-time PCR was utilized to measure miRNA 155 expression and FOXP3 and RORγ mRNA levels in blood samples.
  • The Th17/Treg cell ratio was calculated to assess immune balance.

Main Results:

  • Significantly higher expression of miRNA 155 was observed in atopic dermatitis patients compared to controls (P=0.0040).
  • FOXP3 mRNA expression was statistically higher in patients (P=0.0057), leading to a smaller Th17/Treg ratio (P=0.0012).
  • The ratio of miRNA 155 to Th17/Treg cells was significantly larger in the atopic dermatitis group (P=0.0002).

Conclusions:

  • Elevated miRNA 155, FOXP3, and RORγ responses may indicate immune dysregulation in atopic dermatitis.
  • While direct correlation is not proven, miR-155 and the Th17/Treg ratio appear interconnected in atopic dermatitis.
  • These findings contribute to understanding the molecular mechanisms underlying atopic dermatitis.

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