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Isolation and reconstitution of the high-affinity choline carrier
M Knipper1, I Boekhoff, H Breer
1University Stutgart-Hohenheim, Institute for Zoophysiology, FRG.
FEBS Letters
|March 13, 1989
Abstract:
Monoclonal antibodies, which block the high-affinity uptake of choline in synaptosomal ghosts, have been used to purify a membrane polypeptide (80 kDa) from insect synaptosomal membranes. This isolated protein was found to catalyse the sodium-dependent, hemicholinium-sensitive accumulation of choline after reconstitution into liposomes, thus, apparently represents the high-affinity choline transporter.