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Related Experiment Videos

Coordinate decrease of tissue insulinlike growth factor I posttranscriptional alternative mRNA transcripts in

J A Fagin1, C T Roberts, D LeRoith

  • 1Department of Medicine, Cedars-Sinai Medical Center, UCLA School of Medicine 90048.

Diabetes
|April 1, 1989
PubMed
Summary
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Diabetes mellitus significantly reduces insulin-like growth factor I (IGF-I) gene expression in rats. Insulin therapy can restore IGF-I mRNA levels, suggesting a role for IGF-I in diabetic growth retardation.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Metabolic Research

Background:

  • Diabetes mellitus is characterized by metabolic dysregulation.
  • Insulin-like growth factor I (IGF-I) plays a crucial role in growth and metabolism.
  • The impact of diabetes on IGF-I gene expression requires further elucidation.

Purpose of the Study:

  • To investigate the effect of excess growth hormone (GH) on rat IGF-I gene expression in streptozocin-induced diabetes.
  • To quantify variant IGF-I mRNA species (IGF-Ia and IGF-Ib) in diabetic and non-diabetic rats.
  • To assess the influence of insulin therapy and chronic GH excess on IGF-I mRNA levels.

Main Methods:

  • Streptozocin-induced diabetes mellitus model in rats.
  • Solution hybridization/RNase protection assay to quantify IGF-I mRNA variants.

Related Experiment Videos

  • Utilized somatomammotropic tumors for chronic GH excess induction.
  • Administered insulin therapy to assess its restorative effects.

    • Main Results:

    • IGF-Ia and IGF-Ib mRNA levels were markedly decreased in the liver, kidney, and lung of diabetic rats.
    • Chronic GH excess did not normalize IGF-I mRNA content in diabetic animals.
    • Insulin treatment for one week restored basal and GH-stimulated IGF-I mRNA levels towards normal.
    • The ratio of IGF-Ia to IGF-Ib mRNA remained constant, indicating posttranscriptional splicing is not regulated in these conditions.

    Conclusions:

    • Experimental diabetes profoundly decreases circulating IGF-I levels and tissue IGF-I gene expression.
    • Diminished IGF-I availability may contribute to growth retardation observed in uncontrolled diabetes.
    • Insulin therapy can reverse the suppressive effects of diabetes on IGF-I gene expression.