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Updated: Feb 16, 2026

Fabricating Cotton Analytical Devices
Published on: August 30, 2016
Photofading in cotton fibers dyed using red, yellow, and blue direct dyes during examination with
Amanda L Forster1, Julie L Bitter2, Samuel Rosenthal3
1National Institute of Standards and Technology Material Measurement Laboratory, 100 Bureau Dr. M/S 8102 Gaithersburg, MD 20899-8102, corresponding.
Abstract:
Microspectrophotometry (MSP) is a rapid, nondestructive technique for the analysis of color in textile fibers. This technique combines microscopy and ultraviolet (UV)/visible (Vis) spectroscopy, allowing for very small colored samples, like dyed textile fibers, to be analyzed directly and thereby eliminates the need for time consuming and destructive extractions. While MSP is generally accepted to be a nondestructive evaluation method, a loss of color during analysis, or photofading can occur. In this work, cotton fabric dyed with blue, yellow, and red direct dyes at different concentrations. Dye photofading during MSP examination was investigated by measuring the absorbance at a specific position on the fibers from these fabrics, periodically over the course of 30 minutes. Visible color loss and a reduction in absorbance was observed for all three colors, but was most pronounced for the fibers dyed red. A major goal of this study is to increase awareness of the photofading phenomenon when analyzing cotton fibers using MSP.

