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Updated: Feb 16, 2026
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
miR-141-3p suppresses proliferation and promotes apoptosis by targeting GLI2 in osteosarcoma cells
Nan Wang1, Pengcheng Li1, Wei Liu2
1The 4th Department of Orthopedic Surgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, Liaoning 110024, P.R. China.
Abstract:
MicroRNAs (miRNAs) have been reported as key regulators in numerous diseases including osteosarcoma. The function of microRNA-141-3p (miR-141-3p) and whether this function is achieved by regulation of GLI family zinc finger 2 (GLI2) in osteosarcoma remain unclear. In the present study, we found decreased expression of miR-141-3p, but increased expression of GLI2 in osteosarcoma tissues and cell lines. In addition, we demonstrated a negative correlation between miR-141-3p and GLI2. Furthermore, we revealed that elevation of miR-141-3p resulted in a marked inhibition of proliferation and promotion of apoptosis as well as an obviously decrease in GLI2 in osteosarcoma cell lines. Furthermore, we determined that GLI2 is a target of miR-141-3p by a constructed luciferase assay. In addition, we showed that miR-141-3p could negatively regulate GLI2 and its downstream parathyroid hormone-related protein 1 (PTHRP1). Finally, through a series of antisense experiments we confirmed that the effect of miR-141-3p on proliferation and apoptosis was achieved through the GLI2 pathway in osteosarcoma cells. The findings of the present study may provide a new target for treating osteosarcoma.
Insights
MicroRNA-141-3p (miR-141-3p) is downregulated in osteosarcoma and inhibits cancer cell proliferation by targeting GLI family zinc finger 2 (GLI2). Restoring miR-141-3p may offer a new therapeutic strategy for osteosarcoma.
Area of Science:
- Molecular Biology
- Oncology
- Biochemistry
Background:
- MicroRNAs (miRNAs) are crucial regulators in various diseases, including osteosarcoma.
- The specific role of microRNA-141-3p (miR-141-3p) and its target, GLI family zinc finger 2 (GLI2), in osteosarcoma pathogenesis is not fully understood.
Purpose of the Study:
- To investigate the expression levels of miR-141-3p and GLI2 in osteosarcoma.
- To elucidate the functional relationship between miR-141-3p and GLI2 in osteosarcoma cells.
- To determine if miR-141-3p exerts its effects through the regulation of GLI2.
Main Methods:
- Quantitative real-time PCR to measure miRNA and mRNA expression.
- Western blotting to assess protein levels.
- Luciferase reporter assays to confirm direct targeting.
- Cell proliferation and apoptosis assays.
- Antisense oligonucleotide experiments.
Main Results:
- Osteosarcoma tissues and cell lines exhibited decreased miR-141-3p expression and increased GLI2 expression.
- A negative correlation was observed between miR-141-3p and GLI2 levels.
- Upregulation of miR-141-3p suppressed osteosarcoma cell proliferation and induced apoptosis, accompanied by reduced GLI2 expression.
- GLI2 was validated as a direct target of miR-141-3p.
- miR-141-3p negatively regulated GLI2 and its downstream target, parathyroid hormone-related protein 1 (PTHRP1).
- The anti-proliferative and pro-apoptotic effects of miR-141-3p were mediated via the GLI2 pathway.
Conclusions:
- miR-141-3p acts as a tumor suppressor in osteosarcoma by targeting GLI2.
- The miR-141-3p/GLI2 axis plays a significant role in regulating osteosarcoma cell behavior.
- Targeting the miR-141-3p/GLI2 pathway presents a potential therapeutic avenue for osteosarcoma treatment.
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