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Updated: Feb 16, 2026

Analyzing Mitochondrial Morphology Through Simulation Supervised Learning
Published on: March 3, 2023
Inactivation of cardiolipin synthase triggers changes in mitochondrial morphology
Ayaka Matsumura1, Jun Higuchi1, Yasunori Watanabe2
1Department of Life Science, Rikkyo University, Tokyo, Japan.
Abstract:
Mitochondrial tubular structures are maintained by a balance between membrane fusion and fission that is regulated by various factors, including Drp1 and mitofusin/fzo-1. Here we report the role of cardiolipin (CL) synthase in the regulation of mitochondrial morphology. Knockdown of CL synthase induced mitochondrial elongation in nematode and human cells. Knockdown of both nematode cardiolipin synthase and drp-1 or fzo-1 suggested that knocking down CL synthase decreases mitochondrial division. Mass spectrometric analysis of human CL synthase-knocked down cells revealed a decreased amount of CL and an accumulation of phosphatidylglycerol, a CL precursor. Knockdown of other genes involved in CL synthesis did not influence mitochondrial morphology. Thus, mitochondrial elongation may result from the accumulation of phosphatidylglycerol rather than decreased CL.
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