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Interaction of Lyme disease spirochetes with cultured eucaryotic cells
1Department of Microbiology and Immunology, Bowman Gray School of Medicine, Winston-Salem, North Carolina 27103.
Infection and Immunity
|April 1, 1989
Abstract:
The association of the Lyme disease spirochete, Borrelia burgdorferi, with cultured human endothelial cells was investigated. Attachment was time and temperature dependent, with optimal adherence occurring after 4 h of incubation at 37 degrees C. Pretreatment of borreliae with heat, immune human serum, or monoclonal antibodies directed against outer surface protein B (OspB) reduced the attachment of organisms to host cell monolayers. These results suggest that the adherence of B. burgdorferi may be mediated, at least in part, by borrelial surface proteins.