Related Experiment Video

Updated: Feb 16, 2026

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis
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Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

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Genipin suppresses colorectal cancer cells by inhibiting the Sonic Hedgehog pathway

Bo Ram Kim1, Yoon A Jeong1, Yoo Jin Na2

  • 1Department of Oncology, Korea University Guro Hospital, Seoul, Republic of Korea.

Oncotarget
|December 20, 2017
PubMed

Insights

Genipin, derived from Gardenia jasminoides, inhibits colorectal cancer (CRC) cell growth by suppressing the Hedgehog pathway. This natural compound induces apoptosis via the Hedgehog/p53/NOXA signaling pathway, offering a novel therapeutic strategy for CRC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Genipin, from Gardenia jasminoides, shows anti-cancer properties in various cancers.
  • Its effect on colorectal cancer (CRC) proliferation and underlying mechanisms remain uncharacterized.

Purpose of the Study:

  • To investigate the anti-proliferative effects of genipin on colorectal cancer (CRC) cells.
  • To elucidate the molecular mechanisms, particularly the role of the Hedgehog signaling pathway, in genipin-induced apoptosis in CRC.

Main Methods:

  • Cell proliferation assays were performed on CRC cells treated with genipin.
  • Western blotting and ubiquitination assays were used to analyze protein levels and interactions.
  • A xenograft tumor model was employed to assess genipin's in vivo efficacy.

Main Results:

  • Genipin significantly inhibited CRC cell proliferation and suppressed the Hedgehog signaling pathway.
  • Genipin treatment led to increased p53 and NOXA protein levels, mediating apoptosis.
  • In vivo studies confirmed that genipin suppressed tumor growth by inactivating the Hedgehog pathway.

Conclusions:

  • Genipin induces apoptosis in colorectal cancer cells through the Hedgehog/p53/NOXA signaling pathway.
  • This study reveals a novel mechanism for genipin's anti-cancer activity, highlighting its potential therapeutic value for CRC.
Keywords:
GLI1Hedgehog pathwayNOXAgenipinubiquitin

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