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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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Inner filter effect-based fluorescent sensing systems: A review.

Shuai Chen1, Yong-Liang Yu2, Jian-Hua Wang2

  • 1College of Life and Health Sciences, Northeastern University, Shenyang 110169, China.

Analytica Chimica Acta
|December 20, 2017
PubMed
Summary
This summary is machine-generated.

Inner filter effect (IFE) is now a key spectroscopic technique for chemical and biosensing, offering a flexible alternative to FRET. This review details IFE sensing strategies, materials, and applications for detecting diverse analytes.

Keywords:
FluorescenceInner filter effectSensing applicationSpectroscopic technique

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Area of Science:

  • Spectroscopic techniques
  • Chemical sensing
  • Biosensing

Background:

  • Inner filter effect (IFE) was historically viewed as a fluorescence measurement error.
  • IFE has evolved into a significant non-irradiation energy conversion model in spectroscopy.
  • IFE offers advantages over traditional Forster Resonance Energy Transfer (FRET) in sensing applications.

Purpose of the Study:

  • To provide a comprehensive review of the state-of-the-art in IFE-based fluorescent sensing systems.
  • To detail the sensing strategies, essential conditions, and material choices for IFE systems.
  • To highlight the applications of IFE in detecting various analytes and discuss its benefits and limitations.

Main Methods:

  • Review of recent advancements in Inner Filter Effect (IFE) based fluorescent sensing.
  • Analysis of sensing strategies, including material selection and optimal conditions.
  • Compilation of applications for detecting ionic species, small molecules, and macromolecules.

Main Results:

  • IFE-based fluorescent systems are flexible and straightforward compared to FRET.
  • IFE finds wide applications in chemical sensing and biosensing.
  • The review covers sensing strategies, conditions, materials, and applications for diverse analytes.

Conclusions:

  • IFE is a powerful and versatile tool for fluorescent sensing.
  • IFE-based systems offer a promising alternative to existing sensing methodologies.
  • Critical discussion of IFE benefits and limitations provides insights for future development.