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Continuous electromembrane extraction coupled with mass spectrometry - Perspectives and challenges.

David Fuchs1, Cristina Román Hidalgo2, Maria Ramos Payán3

  • 1Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100 Copenhagen, Denmark; Department of Medical Biochemistry and Biophysics, Karolinska Insitute, Scheeles Väg 2, 17177 Stockholm, Sweden.

Analytica Chimica Acta
|December 20, 2017
PubMed
Summary

Continuous electromembrane extraction (c-EME) coupled with mass spectrometry (MS) enables real-time monitoring of in-vitro drug metabolism. This technique efficiently extracts drugs and metabolites, preventing interference from proteins and salts in mass spectrometry analysis.

Keywords:
Continuous extractionElectromembrane extraction (EME)In-vitro drug metabolismMicro-chipSample preparation

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • In-vitro drug metabolism studies are crucial for drug development.
  • Traditional methods for analyzing drug metabolism can be time-consuming and complex.
  • Matrix effects from proteins and salts often interfere with direct mass spectrometry analysis.

Purpose of the Study:

  • To discuss the principles and applicability of continuous electromembrane extraction (c-EME) coupled directly to mass spectrometry (MS).
  • To highlight the use of c-EME-MS for on-line and real-time monitoring of in-vitro drug metabolism.
  • To explain how c-EME-MS avoids proteins and salts from reaction mixtures entering the mass spectrometer.

Main Methods:

  • Continuous electromembrane extraction (c-EME) using a supported liquid membrane (SLM).
  • Application of an external electrical field across the SLM to facilitate extraction.
  • Direct coupling of the c-EME system to mass spectrometry (MS) for continuous analysis.

Main Results:

  • Demonstration of c-EME-MS for simultaneous extraction and analysis of parent drugs and metabolites.
  • Successful separation of drug compounds from interfering matrix components like proteins and salts.
  • Real-time monitoring of drug metabolism kinetics, showing parent drug decline and metabolite formation.

Conclusions:

  • Continuous electromembrane extraction coupled to mass spectrometry is a powerful tool for on-line, real-time in-vitro drug metabolism studies.
  • The technique offers significant advantages in sample preparation by removing interfering substances.
  • Further development of c-EME-MS systems holds promise for advancing drug discovery and development processes.